COMPARATIVE EVALUATION OF ARGEMONE MEXICANA AND EUCALYPTUS GLOBULUS COMBINATION AND CHENOPODIUM MURALE AND RHIZOMES OF RUMEX ABYSSINICUS COMBINATIONS WITH STANDARD ANTISEPTICS ON WOUND HEALING IN ALBINO MICE

Abstract

This experimental study was carried out at Mekelle university collage of health sciences, from August 2023 to February 2024 to evaluate and compare the wound healing activity of the crude leaf extract of medicinal plant combinations of Argemone mexicana and Eucalyptus globulus, and Chenopodium murale and Rhizomes of R. abyssinicus versus 0.2% Nitrofurazone on wound healing in albino mice. A total of 12 mice were categorized into four groups (G).Mice in G-I (negative control) were treated with simple ointment; mice in G-II were treated with 10% crud extract combination of (5% C. m + 5% R of R a.); mice in G-III were treated with Nitrofurazone 0.2% (positive control); and mice in G-IV were treated with 10% crud extract combination of (5% A. m + 5% E. g).wound healing activity was evaluated based on wound contraction/gap filled and histopathological changes. In this study 10% crud extract combination of 5% A. m + 5% E. g and 10% crud extract combination of 5% C. m + 5% R of R. a exhibited significantly increased (p<0.01) wound contraction rate, shorter epithelialization time, as well as increased collagen deposition, fibroblast proliferation and vascularization as evidenced by histopathological analysis. There was no significant differences (p>0.05) in the ability to cure wounds between 10% crud extract combination of 5% A. m + 5% E. g and 10% crud extract combination of 5% C. m + 5% R of R. a. Similarly, there was no significance difference (p>0.05) between 10% crud extracted combination of 5% C. m + 5% R of R. a and Nitrofurazone 0.2% but the rest significance (p<0.05). Except, complete wound closure was seen in the 10% crud extract combination of 5% A. m + 5% E. g and 10% crud extract combination of 5% C. m + 5% R of R. a and in the 0.2% Nitrofurazone treated groups in the 11th,13th and 15th days of the wound healing time, respectively. The findings of the present study supported the traditional use of these medicinal plants as a wound healing agent through its activity in increasing wound contraction as well as reduction in the epithelization time. However, further study is recommended to evaluate the wound healing effect based on the gab of this research, phytochemical analysis and tensile strength of Argemone mexicana, Eucalyptus globulus, Chenopodium murale and Rhizomes of r. abyssinicus at various combinations and at different solvent fractions