Browsing by Author "Tesfaye Bekele Kassa"

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    ISOLATION AND MOLECULAR IDENTIFICATION OF PASTEURELLA AND MANNHEMIA FROM PNEUMONIC PASTEURELLOSIS SUSPECTED SHEEP AND GOATS IN BISHOFTU TOWN AND THE SURROUNDING RURAL KEBELES OF ADA’A DISTRICT, OROMIA REGION, ETHIOPIA
    (Mekelle University, 2024-02-28) Tesfaye Bekele Kassa
    Respiratory diseases are a major concern for sheep and goats in Ethiopia, particularly in the highland areas where they are reared widely. A cross-sectional study was conducted from February to June 2021 to isolate and molecularly confirm Mannheimia haemolytica and Pasteurella multocida from pneumonic pasteurellosis suspected sheep and goats in Bishoftu Town and the surrounding Rural Kebeles of Ada’a Districts of Oromia Region, Ethiopia. A purposive sampling method was applied to select study animals from those brought to the Veterinary Teaching Hospital of the College of Veterinary Medicine and Agriculture of Addis Ababa University for clinical treatments. Nasal swabs from shoat with suspected pneumonic pasteurellosis were collected and the target bacteria were isolated and identified through standard bacteriological methods using phenotypic features and biochemical tests. Molecular confirmation of isolates was performed through amplification of virulence-associated genes, PHSSA (Pasteurella haemolytica serotypespecific antigen) and Rpt2, of Mannheimia haemolytica and universal gene, KMT1 of Pasteurella multocida using conventional Polymerase Chain Reaction (cPCR). Consequently, from 56 animals (43 sheep and 13 goats), 29 (51.7%), (25 sheep and 4 goats) presumptive isolates of M. hemolytica, and 11 (19.6%) P. multocida were recovered. Out of this, five isolates were positive for either of the two virulence genes of M. haemolytica (Rpt2=four; PHSSA=one) using PCR, but no isolate was confirmed for its universal gene from P. multocida presumptive isolates. From the PCR-positive animals (n=5), four were sheep and one was a goat. The study found that M. haemolytica is a significant bacteria associated with pneumonic pasteurellosis in sheep and goats in the area, highlighting the need for a vaccine targeting these bacteria or its antigens. However, other contributing factors like viral and parasitic agents should also be considered. Further research to identify and characterize these agents in diverse geographical areas and the development of polyvalent respiratory disease vaccines are recommended to address the issue at both the local and national levels