Browsing by Author "Tilahun Arefe Menasibo"

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    ANTI DIABETIC ACTIVITY STUDY ON ROOT AND LEAF EXTRACTS OF MAERUA SUBCORDATA USING RAT MODEL
    (Mekelle University, 2023-11-21) Tilahun Arefe Menasibo
    Diabetes mellitus is a chronic condition that has become a significant global health care issue. The disease affects both humans and animal. The current anti-diabetic drugs' limited efficacy, serious side effects, and constrained availability problems still need to be overcome. As a result, many people prefer to use traditional treatments, and Maeruasubcordata is one of medicinal plants traditionally used to treat diabetes. To study the potential efficacy of M. subcordata as alternative therapies for the management of diabetes on rat model.25 Sprague Dawley (SD) Rats, weighing 140 g (20%) and aged 6 to 8 weeks, were used in this investigation. Five experimental groups were used. Group I (DTM): diabetic treatments with metformin (300 mg/kgbw); Group II (DTL): diabetic treatments with leaf extract (300 mg/kgbw); Group III (DLR): diabetic treatments with root extract (300 mg/kgbw); Group IV (DC): diabetic control (Diabetic, Vehicle control, DW, 10 mL/kg) (positive control); and Group V(NC): normal control (non-diabetic control). After the anti-diabetic study was performed for 28 days, fasting blood Glucose (FBG) and Body weight (BW) were measured in various groups at the specific days of (0, 7, 14, 21, and 28).Moreover, rats were ethically sacrificed, and the organs (liver, kidney, and spleen) were histopathological examined and compared to a normal control. In comparison with the control, treatment with M.subcordats leave and root extracts (300mg/kg) revealed significantly lowered fasting blood glucose (P<0.05) level. However, treatment of the extracts did not show statistically significant reduction in organ and total body weight of the rats (P>0.05). Histhopathological examination showed that M. subcordats leaf and root (300mg/kg b.w.) treated rats had mild microscopic lesions as compared to the untreated diabetic rats while these lesions were comparable to the lesions developed by the standard drug treated rats. This extract treatment revealed that there was an indication of healing processing. Metformin and the tested extracts showed no significant effect on body weight loss, but they do significantly lowered FBGL (P<0.05). Extracts may be employed as a medicinal agent in the management of DM of the hyperglycemic effect M. Subcordata may therefore be clinically helpful in the management of diabetics and in the defense of ß -cells against streptozotocin.