College of Veterinary Sciences
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Item FORMULATION OF USEFULL LOCAL ANESTHESIA FROM DATURA STRAMONIUM USING ALBINO MICE(Mekelle University, 2024-02-25) Mengstom G/herAn experimental study was conducted from August to February 2024 to formulate a useful local anesthesia from D. stramonium using albino mice. In this study16 adult healthy albino mice were used. After collection the plant the leaves of this plant were subjected for drying under shade of at room temperature. The dried leaves were grounded to coarse powder then, dissolved in 70% ethanol and filtered with bright man filter paper. It was subjected to drying hot oven to evaporate the solvent and the crude extract was again dissolved in sterile distilled water for anesthetic formulation. A total of 16 mice were randomly grouped into four groups to see anesthetic effect. (G-I= injected with 0.9% saline water; G-II= treated with the concentration of 5mg/ml extract of D. stramonium; G-III= treated with 7mg/ml of D. stramonium G-IV-treated with the standard drug lidocaine2%, each group containing four mice. In this study, ,Group One (Group: I), mice injected with 0.9% saline water served as negative control and mice treated with lidocaine2% served as (positive control) for comparison of the anesthetic activity of D. stramonium. Data analysis were done using SPSS version 20, and the results were statistically analyzed using one-way ANOVA and the Post hoc Tuckey's test. Results were presented as mean standard error of the mean, with P< 0.05 statistical significance and p>0.05 statistical insignificance. Administration of the leaf extract of D.stramoniumat concentration of 5mg/ml and 7mg/ml exhibited significantly increased (p<0.01) tail flick reaction time as compared to the mice group treated with saline water. There were no significant differences in the tail flick reaction between the concentration of 5mg/ml and 7mg/ml of the extract (p>0.05).Unlike to this there was significantly increased anesthetic activity (p<0.01) of the mice group treated with lidocaine2% compared to the other groups,. The findings of the present study use of D. stramonium leaf extract as anesthetic agent in mice is found to be positive, at a concentration of 5mg/ml and 7mg/ml. Therefore, this experiment supported the traditional use of the plant as local anesthetic agent. However, further study is recommended to evaluate the anesthetic effect of D.stramonium at various dose formulations and it should also be evaluated with different solvent fractions.